Application

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

Cell Line Origin

Cartilage

Components

Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

General description

Feline Chondrocytes, CnC, are derived from normal feline articular cartilage where they produce and maintain the extracellular matrix of cartilage.  Following monolayer culturing, FC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes that allow them to sustain normal proteoglycans production and retention.  Because of the prevalence of degenerative joint disease (DJD) in geriatric cats, FC can be a useful tool for veterinary practice.

Preparation Note

1st passage, >500,000 cells in Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the FC Culture Protocol.